PL-SAX / SCX for Biomolecules

PL-SAX / SCX for Prep to Process from Agilent Technologies

These rigid, strong ion-exchange materials are extremely hydrophilic and are designed for purification of biomolecules. The PL-SAX and PL-SCX materials are totally polymeric and are chemically and thermally stable over a full range of HPLC conditions. The strong ion-exchange functionalities, covalently linked to a chemically stable polymer, facilitate ion-exchange purifications over a wider pH range. This stability can be exploited for column sanitation and clean-up. Thermal stability also enables the use of denaturing conditions and stabilizing/solubilizing agents for the purification of target compounds, as encountered in the purification of synthetic oligonucleotides with self-complementary sequences.

Both the 1000 Å and 4000 Å wide-pore materials are mechanically stable and robust and can be operated over a wide range of linear velocities, with fast loading of dilute solutions and wash cycles. HPLC flow rates and rapid equilibration reduces purification cycle times.

Packing in dynamic axial compression (DAC) column hardware is straightforward and high efficiency columns are achieved with excellent reproducibility and lifetimes. The 1000 Å pore size is for high-capacity purifications and the 4000 Å gigaporous particles with improved mass transfer are intended for large biomolecules and high-speed, high-resolution purifications.