Immobilised
Immobilised Chiral Columns from Daicel
CHIRALPAK IA, IB, IC, ID, IE and IF HPLC and SFC columns are a newer generation of chiral stationary phases (CSPs), in which the polysaccharide chiral selector has been immobilized on to a wide pore silica matrix. This confers universal solvent compatibility on these highly selective chiral stationary phases, without compromising phase stability. The broader range of solvents used as eluents, introduces new selectivity profiles, improved productivity and additional robustness compared with traditional coated polysaccharide phases.
The immobilized product line has been extended by the introduction of 3µm versions of the CHIRALPAK IA, IB, IC, ID, IE and IF phases (CHIRALPAK IA-3, IB-3, IC-3, ID-3, IE-3 and IF-3). These columns are intended for high speed, high efficiency separations of enantiomers and offer the same selectivity and stability characteristics as their 5µm immobilized counterparts. Methods can be transferred directly from 5µm to 3µm particle size columns.
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- Features
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- Immobilized chiral selector
- Broad application range
- Universal solvent compatibility – phases suitable for both NP and RP applications
- Higher screening success rate
- Robustness and extended durability
Wide Solvent Compatibility – The immobilization of the selector confers a wide solvent compatibility to these highly selective chiral stationary
phases, without compromising phase stability. This is in contrast to traditional, coated polysaccharide phases which have restricted solvent compatibility
due to solubility of the polymer coating in certain solvents, including chloroform, methylene chloride, ethyl acetate, acetone, THF and DMF. Immobilized
phases can be used in all chromatographic modes: normal- and reversed-phase HPLC, SFC, analytical and preparative, and the same column can be
used with all eluent combinations.Column Regeneration – CHIRALPAK immobilized columns are more robust than their coated analogues. If columns have been used with additives
or with multiple solvent changes, a regeneration procedure may be implemented to eliminate any change in chiral recognition. Please contact Hichrom for
further details.Screening Strategies – Column screening is simpler, faster and more successful using the four main Daicel immobilized phases (IA, IB, IC and ID).
The recommended eluent combinations and typical starting conditions for screening these phases are shown below:Primary screening mixtures Alkane-2-PrOH Alkane/EtOH Alkane/MtBE/EtOH Alkane/DCM/EtOH Typical starting conditions 80:20 80:20 0:98:2 50:50:2 Advised optimization range 99:1 to 50:50 99:1 to 50:50 80:20:0 to 0:40:60 85:15:0 to 0:100:0 When used in alkane/alcohol solvents, the immobilized columns can
separate a significant number of small molecules, combined with the
advantage of speed and ease of injecting in any suitable solvent. When these immobilized
phase columns are used with the set of four primary screening solvents,
the separation success rate can approach 99%. If the desired chiral
separation is not achieved, then an extended secondary screen can be
applied. - Specifications
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Chiral Selectors
CHIRALPAK IA - based on amylose tris(3,5-dimethylphenyl)carbamate (as in CHIRALPAK AD)
CHIRALPAK IB - based on cellulose tris(3,5-dimethylphenyl)carbamate (as in CHIRALCEL OD)
CHIRALPAK IC - based on cellulose tris(3,5-dichlorophenyl)carbamate
CHIRALPAK ID - based on amylose tris(3-chlorophenyl)carbamate
CHIRALPAK IE – based on amylose tris(3,5-dichlorophenyl)carbamate
CHIRALPAK IF – based on amylose tris(3-chloro, 4-methylphenyl)carbamate
- Literature
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Daicel Chiral Column Catalogue
Full range of Daicel Chiral HPLC columns and applications
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