BioPro IEX

YMC BioPro IEX Biochromatography Columns

Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. BioPro IEX columns are specifically designed for separation of proteins, peptides and nucleic acids.

BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. They also show higher binding capacity and higher recovery of biomolecules compared to conventional IEX columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes!

Available phases are:

BioPro IEX QA - porous polymer beads with -CH2N+(CH3)3 charged group

BioPro IEX SP - porous polymer beads with -(CH2)3SO3- charged group

BioPro IEX QF - non-porous polymer beads with -CH2N+(CH3)3 charged group

BioPro IEX SF - non-porous polymer beads with -(CH2)3SO3- charged group

BioPro IEX

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Features
  • Porous or non-porous hydrophilic polymers with low nonspecific adsorption
  • Excellent binding capacity and recovery of biomolecules
  • Very high resolution
  • Also available as bulk media in 10, 20, 30 or 75 µm
Specifications

BioPro IEX QA/SP Specifications

  BioPro IEX QA BioPro IEX SP
Matrix Hydrophilic polymer (polymethacrylate)
Particle Size 5 µm
Pore Size 100 nm / 1000 Å
Charged group -CH2N+(CH3)3 -(CH2)3SO3-
Counter ion Cl- Na+
Ion-exchange capacity 0.075-0.100 meq/ml-resin 0.070-0.095 meq/ml-resin
Dynamic binding capacity 110-150 mg BSA/ml-resin 70-100 mg human IgG/ml-resin
pH range 2 - 12

 

BioPro IEX QF/SF Specifications

  BioPro IEX QF BioPro IEX SF
Matrix Hydrophilic polymer (polymethacrylate)
Particle Size 3, 5 µm
Pore Size Non-porous
Charged group -CH2N+(CH3)3 -(CH2)3SO3-
Counter ion Cl- Na+
Ion-exchange capacity 0.075-0.110 meq/ml-resin 0.230-0.290 meq/ml-resin
Dynamic binding capacity 12-20 mg BSA/ml-resin 10-18 mg human IgG/ml-resin
pH range 2 - 12
Literature