Agilent Bio SEC
Agilent Bio SEC HPLC Columns
Agilent Bio SEC offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow, and are available in a full range of pore sizes and dimensions to ensure the perfect separation every time.
Agilent Bio SEC-3
These columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle.
Available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.
Agilent Bio SEC-5
These columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution.
Available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å.
- Buy
- Features
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- Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
- Sharper peaks, higher resolution, and better protein recovery
- Faster separations than large-particle SEC columns
- Compatibility with most aqueous buffers
- Excellent stability in high-salt and low-salt conditions
- Specifications
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Agilent Bio SEC-3
Pore Size Particle Size MW Range pH Range Max Pressure Flow Rate 100 Å 3 µm 100 - 100,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) 150 Å 3 µm 500 - 150,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) 300 Å 3 µm 5,000 - 1,250,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) Agilent Bio SEC-5
Pore Size Particle Size MW Range pH Range Max Pressure Flow Rate 100 Å 5 µm 100 - 100,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) 150 Å 5 µm 500 - 150,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) 300 Å 5 µm 5,000 - 1,250,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) 500 Å 5 µm 15,000 - 5,000,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) 1000 Å 5 µm 50,000 - 7,500,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) 2000 Å 5 µm >10,000,000 2 - 8.5 240 bar, 3500 psi 0.1 - 1.25 mL/min (7.8 mm id) 0.1 - 0.4 mL/min (4.6 mm id) - Literature
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Agilent Bio SEC-3 Data Sheet
Data sheet for the Agilent Bio SEC-3 columns
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Agilent Bio SEC-5 Data Sheet
Data sheet for the Agilent Bio SEC-5 columns
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Agilent Bio SEC Column Selection Guide
An overview on Agilent's SEC LC column portfolio, new technology, application examples and intrumentation
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Agilent Bio Column Selection Guide
This comprehensive guide will help you find the right column for your characterization workflow. We have also included advice and tips on method development, solvent choice, mobile phase modification, optimization, and many example separations, all to assist you in column selection and method development.
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