Primesep Acidic

Primesep Acidic from SiELC

Primesep^TM HPLC columns are designed for mixed-mode separations and capable of separating  a tremendous range of compounds by different separation modes based only on the mobile phase selection. With an embedded ion-pairing group, the columns require no ion-pairing reagents in the mobile phase to retain and separate ionizable polar compounds. 

Primesep columns are different in the degree they retain ionic compounds. Neutral compounds are retained on all columns similarly. Primesep A, 100, 200 and 300 are cation exchange columns with different strengths of embedded functional groups.  Primesep N is a normal-phase analytical column with embedded acidic groups with a pKa of about 5.  This phase improves retention of basic compounds by a cation-exchange mechanism at pH > 5 and can separate polar compounds by the HILIC mechanism.