Polymer-based Aqueous SEC

Polymer-based Aqueous SEC Columns from Shodex

  • Polymer-based packed columns for aqueous SEC analysis
  • Network or pores on the surface of the packing material works as molecular sieve to separate molecules based on their sizes
  • To separate molecules solely based on their sizes, it requires an analytical condition without any analyte and packing gel interaction
  • The bigger the molecule size, the faster the elution sequence
  • Used for molecular weight or molecular distribution determination of macromolecules and qualification of oligomers

Polymer-based Aqueous SEC All pictures shown are for illustration purpose only. Actual product may vary.

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Features
SB-800 HQ
  • Polymer-based packed columns for aqueous SEC (GFC) analysis
  • Supports a wide range of molecular weight sample analysis
  • The eluent can be replaced with DMF (except SB-802 HQ and SB-807 HQ), enabling the analysis of polar polymers
  • Method using SB-804 HQ or SB-805 HQ for gelatin's mean molecular weight determination is comparable with PAGI method (Ver. 10 Japan)
  • Fulfills USP L38 and L39 requirements
  • SB-802 and 802.5 HQ fulfill USP L25 requirements
  • SB-803 HQ fulfills USP L37 requirements
SB-807 HQ
  • Column for the analysis of water-soluble ultra high molecular weight polymers
  • Large particle-size gel prevents shear degradation of polymers
  • Fulfills USP L38 and L39 requirements
LB-800
  • Polymer-based packed columns for aqueous SEC (GFC) analysis
  • Controlled column bleeding allows its use with light scattering detectors
  • The eluent can be replaced with DMF enabling the analysis of polar polymer
  • LB-805 (exclusion limit: about 4,000,000) newly added to the series
  • Fulfills USP L38 and L39
Specifications

Usable concentration of organic solvents

Product The maximum usable concentration (%)
Methanol Acetonitrile DMF
SB-802 HQ 0 0 0
SB-802.5 HQ, SB-803 HQ 100 75 100
SB-804 HQ~SB-806M HQ 75 75 100
SB-G 6B 75 75 100
SB-807 HQ, SB-807G 30 30 0
LB-803, LB-805, LB-806M, LB-G 6B 100 100 100

The maximum solvent tolerance of SB-2000 series, preparative columns of SB-800 series, is 50% methanol, acetonitrile or DMF (SB-2002 is not tolerant to organbic solvents).

Target molecular weight range and exclusion limit

Use the below tables for reference purposes only when selecting columns

Measured with pullulan (eluent: ultrapure water)

Product Name Target Molecular Weight Range Exclusion Limit
SB-802 HQ 200 - 1000 1000
SB-802.5 HQ 500 - 10,000 10,000
SB-803 HQ 1000 - 100,000 100,000
SB-804 HQ 5000 - 400,000 1,000,000
SB-805 HQ 100,000 - 1,000,000 *(4,000,000)
SB-806 HQ 100,000 - *(20,000,000) *(20,000,000)
SB-806M HQ 500 - *(20,000,000) *(20,000,000)
SB-807 HQ 500,000 - *(500,000,000) *(500,000,000)
LB-803 1000 - 100,000 100,000
LB-805 100,000 - 1,000,000 *(4,000,000)
LB-806M 500 - *(20,000,000) *(20,000,000)

*(  )  Estimated value

Measured with PEG/PEO (eluent: DMF)

Product Name Target Molecular Weight Range
SB-802.5 HQ 100 - 2000
SB-803 HQ 200 - 40,000
SB-804 HQ 500 - 300,000
SB-805 HQ 50,000 - 700,000
SB-806 HQ 70,000 - *(20,000,000)
SB-806M HQ 200 - *(20,000,000)
LB-803 500 - 50,000
LB-805 50,000 - 700,000
LB-806M 200 - *(20,000,000)

*(  )  Estimated value