Ion Exchange

Ion Exchange HPLC Columns from Shodex

A selection of both strong and weak anion and cation exchange columns are available for a wide range of applications.

Separation is based on electrostatic interactions between the ion exchanger and ionic solutes
The mobile phase of choice should have a sufficient buffering capacity at the pH that produces the largest charge differences between the analyte of interest
The elution position is optimized by varying the pH, salt concentration, and/or ionic strength of the mobile phase

All pictures shown are for illustration purpose only. Actual product may vary.

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Features

Anion Exchange

QA-825	Suitable for analysing relatively high molecular weight compounds: proteins, peptides, DNA and RNA Usuable in a wide pH range from pH 2 to 12 QA-825 fulfills USP L23 requirements
DEAE3N-4T	Non-porous base material For rapid analysis
DEAE-2B	Non-porous base material Can be used with UHPLC (available under hyperbaric conditions up to 30MPa)
ES-502N 7C	Compared to IEC series columns, polyvinyl alcohol is used as base material and this offers different separation pattern Low hydrophobic interaction of proteins allows analysis under mild conditions
WA-624	Suitable for anion exchange analysis of low molecular weight compounds such as nucleotides

Cation Exchange

SP825 CM-825	Suitable for analysing relatively high molecular weight compounds: proteins, peptides, DNA and RNA Usuable in a wide pH range from pH 2 to 12
SP-420N	Non-porous base material For rapid analysis
SP-2B	Compared to IEC series columns, polyvinyl alcohol is used as base material and this offers different separation pattern Low hydrophobic interaction of proteins allows analysis under mild conditions
P-421S	Column for amino acid analysis by cation exchange mode Provides simultaneous analysis of different amino acids Fulfills USP L22 and L58 requirements

Specifications

Strong Anion Exchange

Part Number	Name	Functional Group	Base Material	Ion Exchange Capacity	Particle Size	Pore Size	Shipping Solvent
F6110011	IEC QA-825	Quaternary ammonium	Polyhydroxymethacrylate	0.45 meq/g	12 µm	5000 Å	50mM Na₂S0₄ aq

Weak Anion Exchange

Part Number	Name	Functional Group	Base Material	Ion Exchange Capacity	Particle Size	Pore Size	Shipping Solvent
F6118255	IEC DEAE-825	Diethylaminoethyl	Polyhydroxymethacrylate	0.6 meq/g	8 µm	5000 Å	50mM Na₂S0₄ aq
F6112100	IEC DEAE3N-4T	Diethylaminoethyl	Polyhydroxymethacrylate	0.4 meq/g	2.5 µm	-	H₂O
F7640002	Asahipak ES-502N 7C	Diethylaminoethyl	Polyvinyl alcohol	0.55 meq/g	9 µm	2000 Å	50mM 1,3-Diaminopropane + 50mM NaCl (pH 10)
F6356240	AXpak WA-624	Diethylaminoethyl	Polyhydroxymethacrylate	1.2 meq/g	10 µm	2000 Å	0.1M Sodium phosphate buffer (pH 3)/CH₃CN = 80/20
F6112110	PIKESS DEAE-2B	Diethylaminoethyl	Polyhydroxymethacrylate	0.4 meq/g	2.5 µm	-	H₂0

Strong Cation Exchange

Part Number	Name	Functional Group	Base Material	Ion Exchange Capacity	Particle Size	Pore Size	Shipping Solvent
F6118250	IEC SP-825	Sulfopropyl	Polyhydroxymethacrylate	0.4 meq/g	8 µm	5000 Å	50mM Na₂S0₄ aq
F6113000	IEC SP-420N	Sulfopropyl	Polyhydroxymethacrylate	0.3 meq/g	2.5 µm	-	20mM Sodium acetate buffer + 0.5M Na₂SO₄ (pH5)
F6113110	PIKESS SP-2B	Sulfopropyl	Polyhydroxymethacrylate	0.3 meq/g	2.5 µm	-	20mM Sodium acetate buffer + 0.5M Na₂SO₄ (pH5)

Weak Cation Exchange

Amino Acid Analysis

Part Number	Name	Functional Group	Base Material	Plate Number	Particle Size	Shipping Solvent
F6354211	CXpak P-421S	Sulfo (Na⁺)	Styrene divinylbenzene copolymer	≥3500 TP/column	6 µm	H₂O

Literature